Tom Brown Group

Optimised oligonucleotide substrates to assay XPF ERCC1 nuclease activity for the discovery of DNA repair inhibitors

A. M. Thomas, S. Brolih, J. F. McGouran, A. H. El-Sagheer, D. Ptchelkine, M. Jones P. J. McHugh and T. Brown. Chem. Commun. 55 (78), 11671-11674, 2019.

A hydroxamic-acid-containing nucleoside inhibits DNA repair nuclease SNM1A

William Doherty, Eva-Maria Dürr, Hannah T. Baddock, Sook Y. Lee, Peter J. McHugh, Tom Brown, Mathias O. Senge, Eoin M. Scanlana, Joanna F. McGouran. Organic and Biomolecular Chemistry 17, 8094-8105, 2019.

Radiolabeled oligonucleotides targeting the RNA subunit of telomerase inhibit telomerase and induce DNA damage in telomerase-positive cancer cells

M. R. Jackson, B. M. Bavelaar, P. A. Waghorn, M. R. Gill, A. H. El-Sagheer, T. Brown, M. Tarsounas and K. A. Vallis. Cancer Res. 2019.

Design of thiazole orange oligonucleotide probes for detection of DNA and RNA by fluorescence and duplex melting

P. Klimkowski, S. De Ornellas, D. Singleton, A. H. El-Sagheer and T. Brown. Org. Biomol. Chem. 17, 5943-5950, 2019.

Curtailing their negativity

A. Brown and T. Brown. Nat. Chem. 11 (6), 501-503, 2019.

Synthesis and biophysical properties of carbamate-locked nucleic acid (LNA) oligonucleotides with potential antisense applications

C. Thorpe, S. Epple, B. Woods, A. H. El-Sagheer and T. Brown. Org. Biomol. Chem. 17, 5341-5348, 2019.

Light-Induced Reversible DNA Ligation of Gold Nanoparticle Superlattices

Angela F. De Fazio, Afaf H. El-Sagheer, Jason S. Kahn, Iris Nandhakumar, Matthew Richard Burton, Tom Brown, Otto L. Muskens, Oleg Gang*, and Antonios G. Kanaras*. ACS Nano 13 (5), 5771-5777, 2019.

“Split-and-Click” sgRNA

Lapatrada Taemaitree, Arun Shivalingam, Afaf H. El-Sagheer, Tom Brown. Methods in molecular biology 2162, 61-78, 2019.

An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA

L. Taemaitree, A. Shivalingam, A. H. El-Sagheer and T. Brown. Nat. Commun. 10, 2019.

DNA-Coated Gold Nanoparticles for the Detection of mRNA in Live Hydra Vulgaris Animals

M. Moros, M. Kyriazi, A. H. El-Sagheer, T. Brown, C. Tortiglione and A. G. Kanaras. ACS Appl. Mater. Interfaces 11 (15), 13905-13911, 2018.

Squaramide-Based 5'-Phosphate Replacements Bind to the DNA Repair Exonuclease SNM1A

E. Dürr, W. Doherty, S. Y. Lee, A. H. El-Sagheer, A. Shivalingam, P. J. McHugh, T. Brown and J. F. McGouran. ChemistrySelect 3 (45), 12824-12829, 2018.

Synthesis, oligonucleotide incorporation and fluorescence properties in DNA of a bicyclic thymine analogue

C. P. Lawson, A. F. Fuchtbauer, M. S. Wranne, T. Giraud, T. Floyd, B. Dumat, N. K. Andersen, A. H. El-Sagheer, T. Brown, H. Graden, L. M. Wilhelmsson and M. Grotli. Sci. Rep. 8, 2018.

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation

J. Chen, Y. R. Baker, A. Brown, A. H. El-Sagheer and T. Brown. Chem. Sci. 9, 8110-8120, 2018.

Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above

S. Walsh, A. H. El-Sagheer and T. Brown. Chem. Sci. 9, 7681-7687, 2018.

Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size

Y. R. Baker, J. Chen, J. Brown, A. H. El-Sagheer, P. Wiseman, E. Johnson, P. Goddard and T. Brown. Nucleic Acids Res. 46 (15), 7495-7505, 2018.

Synthesis, Affinity for Complementary RNA and DNA, and Enzymatic Stability of Triazole-Linked Locked Nucleic Acids (t-LNAs).

P. Kumar, L. Truong Y. R. Baker, A. H. El-Sagheer and T. Brown. ACS Omega 3 (6), 6976-6987, 2018.

Replication Fork Reversal during DNA Interstrand Crosslink Repair Requires CMG Unloading

R. Amunugama, S. Willcox, R. A. Wu, U. B. Abdullah, A. H. El-Sagheer, T. Brown, P. J. McHugh, J. D. Griffith and J. C. Walter. Cell Reports 23, 3419-3428, 2018.

Synthesis of Lipid-Carbohydrate-Peptidyl-RNA Conjugates to Explore the Limits Imposed by the Substrate Specificity of Cell Wall Enzymes on the Acquisition of Drug Resistance

M. Fonvielle, A. Bouhss, C. Hoareau, D. Patin, D. Mengin-Lecreulx, L. Iannazzo, N. Sakkas, A. H. El-Sagheer, T. Brown, M. Etheve-Quelquejeu and M. Arthur. Chem. Eur. J. 24 (56), 14911-14915, 2018.

Graphene Oxide-Upconversion Nanoparticle Based Portable Sensors for Assessing Nutritional Deficiencies in Crops

D. Giust, M. I. Lucio, A. H. El-Sagheer, T. Brown, L. E. Williams, O. L. Muskens and A. G. Kanaras. ACS Nano 12 (6), 6273-6279, 2018.

NMR analyses on N-hydroxymethylated nucleobases - implications for formaldehyde toxicity and nucleic acid demethylases

S. Shishodia, D. Zhang, A. H. El-Sagheer, T. Brown, T. D. W. Claridge, C. J. Schofield and R. J. Hopkinson. Org. Biomol. Chem. 16 (21), 4021-4032, 2018.