Synthesis and properties of oligonucleotides containing a cholesterol thymidine monomer

A. Durand and T. Brown. Nucleosides Nucleotides Nucl. Acids 26 (6-7), 785-794, 2007.

Abstract

Highly selective base-pair recognition makes DNA a suitable building block for orderly self-assembled structures. For some applications in nanotechnology DNA complexes need to be fixed onto surfaces. To fulfil this requirement on lipid membranes we have synthesised a thymidine monomer modified with a cholesterol moiety. Solution studies show that the melting temperature (Tm) of the duplex, with adjacent cholesterols on each strand, is much higher than that of the unmodified duplex.

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