Our research focuses on nucleic acid chemistry and its applications.
We synthesize new nucleic acid analogues and study their fundamental properties.
Our research has led to the foundation of three companies, as well as the development of several new technologies in collaboration with industrial partners.
In a paper published today in Nature Communications, we demonstrate a new approach to the chemical synthesis of libraries of sgRNAs for CRISPR applications. Using the method, standard or chemically modified CRISPR sgRNA libraries can be prepared in a single tube by click ligation of an invariant Cas9-binding 79-mer and a library of variable genome-targeting 20-mers. See our blog post for more information: https://chemistrycommunity.nature.com/users/251216-arun-shivalingam/posts/47615-split-and-click-sgrna.
Optimised oligonucleotide substrates to assay XPF ERCC1 nuclease activity for the discovery of DNA repair inhibitors