Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries

S. Matysiak, K. Hellmuth, A. H. El-Sagheer, A. Shivalingam, Y. Ariyurek, M. de Jong, M. J. Hollestelle, R. Out and T. Brown. Org. Biomol. Chem. 16, 48-52, 2018.


DNA encoded ligands are self-assembled into bivalent complexes and chemically ligated to link their identities. To demonstrate their potential as a combinatorial screening platform for avidity interactions, the optimal bivalent aptamer design (examplar ligands) for human alpha-thrombin is determined in a single round of selection and the DNA scaffold replaced with minimal impact on the final design.