Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes

C. M. McKeen, L. J. Brown, J. T. G. Nicol, J. M. Mellor and T. Brown. Org. Biomol. Chem. 1 (13), 2267-2275, 2003.

Abstract

We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.

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