Tom Brown Group

Modular and Automated Synthesis of Oligonucleotide-Small Molecule Conjugates for Cathepsin B Mediated Traceless Release of Payloads

Cheng Jin, Siqi Li, Katherine A. Vallis, Afaf H. El-Sagheer and Tom Brown. RSC Chemical Biology

Synthesis and Properties of Oligonucleotides Containing LNA-Sulfamate and Sulfamide Backbone Linkages

Belma Zengin Kurt, Debashis Dhara, Afaf H. El-Sagheer and Tom Brown. Org. Lett 26 (19), 4137-4141, 2024.

A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes

Chunsen Bai, Piotr Klimkowski, Cheng Jin, Jagannath Kuchlyan, Afaf H. El-Sagheer* and Tom Brown*. Org. Biomol. Chem. 20, 8618-8622, 2022.

An LNA-amide modification that enhances the cell uptake and activity of phosphorothioate exon-skipping oligonucleotides

Ysobel Baker, Cameron Thorpe, Jinfeng Chen, Laura Poller, Lina Cox, Pawan Kumar, Wooi Fang Lim, Lillian Lie, Graham McClorey, Sven Epple, Daniel Singleton, Michael McDonough, Jack Hardwick, Kirsten Christensen, Matthew Wood, James Hall, Afaf El-Sagheer, Tom Brown. Nature Communications 13 (1), 1-11, 2022.

Enzymatic Synthesis of Chemical Nuclease Triplex-Forming Oligonucleotides with Gene-Silencing Applications

Brionna McGorman, Nicolo Zuin Fantoni , Sinead O’Carroll , Anna Ziemele, Afaf H. El-Sagheer, Tom Brown and Andrew Kellett. Nucleic Acids Research 50, 5467-5487, 2022.

Covalently attached intercalators restore duplex stability and splice-switching activity to triazole-modified oligonucleotides

Anna Dysko, Ysobel R. Baker, Graham McClorey, Matthew J. A. Wood, Sabine Fenner, Glynn Williams, Afaf El-Sagheer and Tom Brown. RSC Chemical Biology 3, 765-772, 2022.

Transient DNA binding to gapped DNA substrates links DNA sequence to the single-molecule kinetics of protein-DNA interactions

Rebecca Andrews, Horst Steuer, Afaf H El-Sagheer, Abhishek Mazumder, Hafez el Sayyed, Arun Shivalingam, Tom Brown, Achillefs N Kapanidis. bioRxiv 0 (0), 2022.

The role of chemistry in the success of oligonucleotides as therapeutics

Pawan Kumar and Tom Brown. Beilstein Journal of Organic Chemistry 18, 197-199, 2022.

“Bind, cleave and leave”: multiple turnover catalysis of RNA cleavage by bulge–loop inducing supramolecular conjugates

Bahareh Amirloo, Yaroslav Staroseletz, Sameen Yousaf, David J Clarke, Tom Brown, Harmesh Aojula, Marina A Zenkova, Elena V Bichenkova. Nucleic Acids Res. 50 (2), 651-673, 2022.

Chirality transmission in macromolecular domains

Shankar Pandey, Shankar Mandal, Mathias Bogetoft Danielsen, Asha Brown, Changpeng Hu, Niels Johan Christensen, Alina Vitaliyivna Kulakova, Shixi Song, Tom Brown, Knud J. Jensen, Jesper Wengel, Chenguang Lou & Hanbin Mao . Nature Communications 13, 76, 2022.

An Investigation into the Resistance of Spherical Nucleic Acids against DNA Enzymatic Degradation

Maria-Eleni Kyriazi, Afaf H. El-Sagheer, Igor L. Medintz, Tom Brown, and Antonios G. Kanaras*. Bioconjugate Chem. 33 (1), 219-225, 2022.

Engineering Enzyme-Cleavable Oligonucleotides by Automated Solid-Phase Incorporation of Cathepsin B Sensitive Dipeptide Linkers

Cheng Jin, A. H. EI-Sagheer, Siqi Li, Katherine A. Vallis, Weihong Tan, Tom Brown. Angew. Chem. Int. Ed. e202114016 1, 2022.

Deoxyribonucleic Acid Encoded and Size-Defined pi-Stacking of Perylene Diimides

Jeffrey Gorman, Sarah R. E. Orsborne, Akshay Sridhar, Raj Pandya, Peter Budden, Alexander Ohmann, Naitik A. Panjwani, Yun Liu, Jake L. Greenfield, Simon Dowland, Victor Gray, Seán T. J. Ryan, Sara De Ornellas, Afaf H. El-Sagheer, Tom Brown, Jonathan R. Nitschke, Jan Behrends, Ulrich F. Keyser, Akshay Rao, Rosana Collepardo-Guevara, Eugen Stulz, Richard H. Friend*, and Florian Auras*. Journal of the American Chemical Society 144 (1), 368-376, 2022.

Chemically modified nucleic acids and DNA intercalators as tools for nanoparticle assembly

Angela F. De Fazio, Doxi Misatziou, Ysobel R. Baker, Otto L. Muskens, Tom Brown and Antonios G. Kanaras. Chemical Society Reviews 50, 13410-13440, 2021.

Structure-Based Design of Selective Fat Mass and Obesity Associated Protein (FTO) Inhibitors

Shifali Shishodia, Marina Demetriades, Dong Zhang, Nok Yin Tam, Pratheesh Maheswaran, Caitlin Clunie-O’Connor, Anthony Tumber, Ivanhoe K. H. Leung, Yi Min Ng, Thomas M. Leissing, Afaf H. El-Sagheer, Eidarus Salah, Tom Brown, Wei Shen Aik*, Michael A. McDonough*, and Christopher J. Schofield*. Journal of Medical Chemistry 64 (22), 16609-16625, 2021.

A Click Chemistry Approach to Targeted DNA Crosslinking with cis-Platinum(II)-Modified Triplex-Forming Oligonucleotides

Joseph Hennessy, Bríonna McGorman, Zara Molphy, Nicholas P. Farrell, Daniel Singleton, Tom Brown, Andrew Kellett. Angew. Chem. Int. Ed. 61 (3), 2022.

A New 1,5-Disubstituted Triazole DNA Backbone Mimic with Enhanced Polymerase Compatibility

Sven Epple, Aman Modi, Ysobel R. Baker, Ewa Wegrzyn, Diallo Traoré, Przemyslaw Wanat, Agnes E. S. Tyburn, Arun Shivalingam, Lapatrada Taemaitree, Afaf H. El-Sagheer, and Tom Brown*. Journal of the American Chemical Society 143 (39), 16293-16301, 2021.

Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification

Ysobel R Baker, Liyiwen Yuan, Jinfeng Chen, Roman Belle, Robert Carlisle, Afaf H El-Sagheer, Tom Brown. Nucleic Acids Res. 49 (16), 9042-9052, 2021.

Artificial nucleic acid backbones and their applications in therapeutics, synthetic biology and biotechnology

Sven Epple, Afaf H. El-Sagheer, Tom Brown. Energing topics in life science 5 (5), 691-697, 2021.

Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq

Martin Philpott, Jonathan Watson, Anjan Thakurta, Tom Brown Jr, Tom Brown Sr, Udo Oppermann & Adam P. Cribbs. Nature Biotechnology 39, 1517-1520, 2021.