Tom Brown Group

Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes

X. Ren, A. H. El-Sagheer and T. Brown. Nucleic Acids Res. 2016.

New Technologies for DNA analysis-A review of the READNA Project

S. McGinn, D. Bauer, T. Brefort, L. Dong, A. H. El-Sagheer, A. Elsharawy, G. Evans, E. Falk-Sorqvist, M. Forster, S. Fredriksson, P. Freeman, C. Freitag, J. Fritzsche, S. Gibson, M. Gullberg, M. Gut, S. Heath, I. Heath-Brun, A. J. Heron, J. Hohlbein, R. Ke, O. Lancaster, L. Le Reste, G. Maglia, R. Marie, F. Mauger, F. Mertes, M. Mignardi, L. Moens, J. Oostmeijer, R. Out, J. N. Pedersen, F. Persson, V. Picaud, D. Rotem, N. Schracke, J. Sengenes, P. F. Stahler, B. Stade, D. Stoddart, X. Teng, C. D. Veal, N. Zahra, H. Bayley, M. Beier, T. Brown, C. Dekker, B. Ekstrom, H. Flyvbjerg, A. Franke, S. Guenther, A. N. Kapanidis, J. Kaye, A. Kristensen, H. Lehrach, J. Mangion, S. Sauer, E. Schyns, J. Tost, J. M. L. M. van Helvoort, P. J. van der Zaag, J. O. Tegenfeldt, A. J. Brookes, K. Mir, M. Nilsson, S. Willcocks and I. G. Gut. N. Biotechnol. 33 (3), 311-330, 2016.

Specifically horizontally tethered DNA probes on Au surfaces allow labelled and label-free DNA detection using SERS and electrochemically driven melting

E. Papadopoulou, N. Gale, J. F. Thompson, T. A. Fleming, T. Brown and P. N. Bartlett. Chem. Sci. 7 (1), 386-393, 2016.

The effect of sequence context on the activity of cytosine DNA glycosylases

S. T. Kimber, T. Brown and K. R. Fox. Mol. Biosyst. 106, 353-358, 2015.

The effect of temperature on electrochemically driven denaturation monitored by SERS.

E. Papadopoulou, M. Meneghello, P. Marafini, R. P. Johnson, T. Brown and P. N. Bartlett. Bioelectrochemistry 106, 353-358, 2015.

Programming the assembly of gold nanoparticles on graphene oxide sheets using DNA

A. Heuer-Jungemann, L. Kiessling, E. Stratakis, E. Kymakis, A. H. El-Sagheer, T. Brown and A. G. Kanaras. J. Mater. Chem. C 3, 9379-9384, 2015.

Click DNA and RNA ligation for new biocompatible nucleic acid backbone mimics

A. H. El-Sagheer and T. Brown. Collect. Czech. Chem. Commun. 12, 10-17, 2011.

Reversible Ligation of Programmed DNA-Gold Nanoparticle Assemblies

P. K. Harimech, S. R. Gerrard, A. H. El-Sagheer, T. Brown and A. G. Kanaras. J. Am. Chem. Soc. 137 (29), 9242-9245, 2015.

A triazole linkage that mimics the DNA phosphodiester group in living systems

A. H. El-Sagheer and T. Brown. Q. Rev. Biophys 48 (4), 429-436, 2015.

Synthesis and use of universal sequence probes in fluorogenic multi-strand hybridisation complexes for economical nucleic acid testing

D. J. French, J. A. Richardson, R. L. Howard, T. Brown and P. G. Debenham. Mol. Cell. Probes 29 (4), 228-236, 2015.

Azide and trans-Cyclooctene dUTPs: Incorporation into DNA Probes and Fluorescent Click-Labelling

T. Brown, X. Ren and A. H. El-Sagheer. Analyst 2015.

Reversible Energy-Transfer Switching on a DNA Scaffold

M. Bälter, M. Hammarson, P. Remón, S. Li, N. Gale, T. Brown and J. Andréasson. J. Am. Chem. Soc. 137 (7), 2444-2447, 2015.

Highly Sensitive DNA Sensor Based on Upconversion Nanoparticles and Graphene Oxide

P. Alonso-Cristobal, P. Vilela, A. H. El-Sagheer, E. Lopez-Cabarcos, T. Brown, O. L. Muskens, J. Rubio-Retama and A. G. Kanaras. ACS Appl. Mater. Interfaces 2015.

Strain discrimination of Yersinia pestis using a SERS-based electrochemically driven melting curve analysis of variable number tandem repeat sequences

E. Papadopoulou, N. Gale, S. A. Goodchild, D. W. Cleary, S. A. Weller, T. Brown and P. N. Bartlett. Chem. Sci. 2015.

Using Surface-Enhanced Raman Spectroscopy and Electrochemically Driven Melting to Discriminate Yersinia pestis from Y. pseudotuberculosis Based on Single Nucleotide Polymorphisms within Unpurified Polymerase Chain Reaction Amplicons

E. Papadopoulou, S. A. Goodchild, D. W. Cleary, S. A. Weller, N. Gale, M. R. Stubberfield, T. Brown and P. N. Bartlett. Anal. Chem. 87 (3), 1605-1612, 2015.

Rapid detection of diagnostic targets using isothermal amplification and HyBeacon probes – A homogenous system for sequence-specific detection

R. L. Howard, D. J. French, J. A. Richardson, C. E. O'Neill, M. P. Andreou, T. Brown, D. Clark, I. N. Clarke, J. W. Holloway, P. Marsh and P. G. Debenham. Mol. Cell. Probes 29 (2), 92-98, 2015.

Enhanced H-bonding and pi-stacking in DNA: A potent duplex-stabilizing and mismatch sensing nucleobase analogue

C. Lou, A. Dallmann, P. Marafini, R. Gao and T. Brown. Chem. Sci. 5 (10), 3836-3844, 2014.

Enzymatic Incorporation and Fluorescent Labelling of Cyclooctyne-Modified Deoxyuridine Triphosphates in DNA

X. Ren, M. Gerowska, A. H. El-Sagheer and T. Brown. Bioorg. Med. Chem. 2014.

Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing

X. Chen, A. H. El-Sagheer and T. Brown. Chem. Commun. 50, 7597-7600, 2014.

Force-induced melting of DNA—evidence for peeling and internal melting from force spectra on short synthetic duplex sequences

N. Bosaeus, A. H. El-Sagheer, T. Brown, B. Akerman and B. Norden. Nucleic Acids Res. 42 (12), 8083-8091, 2014.