Click DNA and RNA ligation for new biocompatible nucleic acid backbone mimics

A. H. El-Sagheer and T. Brown. Collect. Czech. Chem. Commun. 12, 10-17, 2011.


We have used the CuAAC reaction (click chemistry) to construct cyclic DNA strands, DNA catenanes, and artificial DNA backbones that can be read through by DNA polymerases. We have also constructed chemically-modified ribozymes up to 100 bases in length that are biochemically active.