Tom Brown Group

An LNA-amide modification that enhances the cell uptake and activity of phosphorothioate exon-skipping oligonucleotides

Ysobel Baker, Cameron Thorpe, Jinfeng Chen, Laura Poller, Lina Cox, Pawan Kumar, Wooi Fang Lim, Lillian Lie, Graham McClorey, Sven Epple, Daniel Singleton, Michael McDonough, Jack Hardwick, Kirsten Christensen, Matthew Wood, James Hall, Afaf El-Sagheer, Tom Brown. Nature Communications 13 (1), 1-11, 2022.

Engineering Enzyme-Cleavable Oligonucleotides by Automated Solid-Phase Incorporation of Cathepsin B Sensitive Dipeptide Linkers

Cheng Jin, A. H. EI-Sagheer, Siqi Li, Katherine A. Vallis, Weihong Tan, Tom Brown. Angew. Chem. Int. Ed. e202114016 1, 2022.

A New 1,5-Disubstituted Triazole DNA Backbone Mimic with Enhanced Polymerase Compatibility

Sven Epple, Aman Modi, Ysobel R. Baker, Ewa Wegrzyn, Diallo Traoré, Przemyslaw Wanat, Agnes E. S. Tyburn, Arun Shivalingam, Lapatrada Taemaitree, Afaf H. El-Sagheer, and Tom Brown*. Journal of the American Chemical Society 143 (39), 16293-16301, 2021.

Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification

Ysobel R Baker, Liyiwen Yuan, Jinfeng Chen, Roman Belle, Robert Carlisle, Afaf H El-Sagheer, Tom Brown. Nucleic Acids Res. 49 (16), 9042-9052, 2021.

Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq

Philpott M, Watson J, Thakurta A, Brown T, Brown T, Oppermann U, Cribbs AP. Nature Biotechnology 39 (12), 1517-1520, 2021.

A Hitchhiker’s Guide to Click-Chemistry with Nucleic Acids

Nicolò Zuin Fantoni, Afaf H. El-Sagheer, and Tom Brown*. Chemical Reviews 121 (12), 7122-7154, 2021.

Consecutive 5'- and 3'-amide linkages stabilise antisense oligonucleotides and elicit an efficient RNase H response

Sven Epple, Cameron Thorpe, Ysobel R. Baker, Afaf H. El-Sagheer, Tom Brown. Chemical Communications 56, 5496-5499, 2020.

Squaramides and Ureas: A Flexible Approach to Polymerase-Compatible Nucleic Acid Assembly

Arun Shivalingam, Lapatrada Taemaitree, Afaf H. El-Sagheer, Tom Brown. Angew. Chem. Int. Ed. 59 (28), 11416-11422, 2020.

Curtailing their negativity

A. Brown and T. Brown. Nat. Chem. 11 (6), 501-503, 2019.

An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA

L. Taemaitree, A. Shivalingam, A. H. El-Sagheer and T. Brown. Nat. Commun. 10, 2019.

Assembly of a biocompatible triazole-linked gene by one-pot click-DNA ligation

M. Kukwikila, N. Gale, A. H. El-Sagheer, T. Brown and A. Tavassoli. Nat. Chem. 9, 1089-1098, 2017.

5-Formylcytosine does not change the global structure of DNA

J. S. Hardwick, D. Ptchelkine, A. H. El-Sagheer, I. Tear, D. Singleton, S. E. V. Phillips, A. N. Lane and T. Brown. Nat. Struct. Mol. Biol. 24, 544-552, 2017.

Molecular Requirements of High-Fidelity Replication-Competent DNA Backbones for Orthogonal Chemical Ligation

A. Shivalingam, A. E. S. Tyburn, A. H. El-Sagheer and T. Brown. J. Am. Chem. Soc. 139 (4), 1575-1583, 2017.

2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA

M. M. Haugland, A. H. El-Sagheer, R. J. Porter, J. Pena, T. Brown, E. A. Anderson and J. E. Lovett. J. Am. Chem. Soc. 2016.

An autonomous molecular assembler for programmable chemical synthesis

W. Meng, R. A. Muscat, M. L. McKee, P. J. Milnes, A. H. El-Sagheer, J. Bath, B. G. Davis, T. Brown, R. K. O'Reilly and A. J. Turberfield. Nat. Chem. 2016.

Reversible Ligation of Programmed DNA-Gold Nanoparticle Assemblies

P. K. Harimech, S. R. Gerrard, A. H. El-Sagheer, T. Brown and A. G. Kanaras. J. Am. Chem. Soc. 137 (29), 9242-9245, 2015.

Transcription of Click-Linked DNA in Human Cells

C. N. Birts, A. P. Sanzone, A. H. El-Sagheer, J. P. Blaydes, T. Brown and A. Tavassoli. Angew. Chem. Int. Ed. 53 (9), 2362-2365, 2014.

Combined nucleobase and backbone modifications enhance DNA duplex stability and preserve biocompatibility

A. H. El-Sagheer and T. Brown. Chem. Sci. 5, 253-259, 2014.

Click Nucleic Acid Ligation: Applications in Biology and Nanotechnology

A. H. El-Sagheer and T. Brown. Acc. Chem. Res. 45 (8), 1258-1267, 2012.

Structure and Dynamics of Triazole-Linked DNA: Biocompatibility Explained

A. Dallmann, A. H. El-Sagheer, L. Dehmel, C. Muegge, C. Griesinger, N. P. Ernsting and T. Brown. Chem. Eur. J. 17 (52), 14714-14717, 2011.