Fast Copper-Free Click DNA Ligation by the Ring Strain Promoted Alkyne-Azide Cycloaddition Reaction
Chem. Commun. 47, 6257-6259, 2011.
Abstract
Templated DNA strand ligation by the ring-strain promoted alkyne–azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.