Efficient RNA synthesis by in vitro transcription of a triazole-modified DNA template

A. H. El-Sagheer and T. Brown. Chem. Commun. 47 (44), 12057-12058, 2011.

This paper was featured on the front cover of ChemComm (Volume 47, issue 44).


A DNA strand containing a triazole phosphodiester mimic is an efficient template for in vitro transcription. This is the first demonstration of transcription through a heavily modified DNA backbone linkage and it suggests that click-ligated DNA could be useful for the direct synthesis of biologically active RNA and proteins.