PNA HyBeacons for analysis of human mutations related to statin-induced myopathy

N. Gale, P. Kocalka, C. Mardle and T. Brown. Artificial DNA: PNA & XNA 2 (3), 1-11, 2011.


Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.