Tom Brown Group

Non-covalent Single Transcription Factor Encapsulation Inside a DNA Cage

R. Crawford, C. M. Erben, J. Periz, L. M. Hall, T. Brown, A. J. Turberfield and A. N. Kapanidis. Angew. Chem. Int. Ed. 52 (8), 2284-2288, 2013.

Abstract

A cage made from DNA can be used to encapsulate a transcription factor (catabolite activator protein, CAP) without covalent attachment. CAP is shown to stably bind inside the DNA cage at a 1:1 ratio by bending one edge to accommodate the protein. Single-molecule fluorescence measurements confirm the orientation of CAP within the cage.