Solid phase click ligation for the synthesis of very long oligonucleotides

J. Qiu, A. H. El-Sagheer and T. Brown. Chem. Commun. 49, 6959-6961, 2013.


Oligonucleotides have been ligated efficiently on solid-phase using CuAAC and SPAAC chemistry to produce up to 186-mer triazole linked DNA products. Multiple sequential ligation reactions can be carried out by using a masked azide approach. This work suggests a novel modular approach to the synthesis of large complex oligonucleotide analogues.