Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA

P. Kumar, A. H. El-Sagheer, L. Truong and T. Brown. Chem. Commun. 53, 8910-8913, 2017.


Oligonucleotides containing internal triazole–3?-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified oligonucleotides, and significantly better than oligonucleotides containing triazole alone. In contrast LNA on the 5?-side of the triazole does not stabilise duplexes. Triazole–LNA confers great resistance towards enzymatic degradation relative to LNA alone.