Redox Capacitive Assaying of C-Reactive Protein at a Peptide Supported Aptamer Interface

J. Piccoli, R. Hein, A. H. El-Sagheer, T. Brown, E. M. Cilli, P. R. Bueno and J. J. Davis. Anal. Chem. 90 (5), 3005-3008, 2018.


Electrochemical immunosensors offer much in the potential translation of a lab based sensing capability to a useful “real world” platform. In previous work we have introduced an impedance-derived electrochemical capacitance spectroscopic analysis as supportive of a reagentless means of reporting on analyte target capture at suitably prepared mixed-component redox-active, antibody-modified interfaces. Herein we directly integrate receptive aptamers into a redox charging peptide support in enabling a label-free low picomolar analytical assay for C-reactive protein with a sensitivity that significantly exceeds that attainable with an analogous antibody interface.