Synthesis and antibody-mediated detection of 2,4-dinitrophenyl (DNP) labelled oligonucleotides
11th International Round Table on Nucleosides, Nucleotides and Their Biological Applications, Leuven, Belgium 1049-1052, 1994.
Abstract
DNP-labelled phosphoramidites have been used to synthesis oligonucleotides with multiple DNP reporter groups. The antibody-mediated detection and the stability of duplexes formed by these labelled oligonucleotides have been studied. A DNP-labelled deoxyuridine triphosphate has also been used to enzymatically incorporate DNP-labels into DNA via the polymerase chain reaction. The use of DNP-labelled primers in the PCR has also been investigated.