Use of Inter-Proton Nuclear Overhauser Effects to Assign the Nuclear Magnetic-Resonance Spectra of Oligodeoxynucleotide and Hybrid Duplexes in Aqueous-Solution

D. G. Reid, S. A. Salisbury, T. Brown, D. H. Williams, J. J. Vasseur, B. Rayner and J. L. Imbach. Eur. J. Biochem. 135 (2), 307-314, 1983.

Abstract

Inter-proton nuclear Overhauser enhancements (NOEs) have been used to assign the aromatic, anomeric and 2' resonances in the 1H nuclear magnetic resonance spectrum of the duplex formed between d(T-C-A-C-A-T) and d(A-T-G-T-G-A). The same techniques have been applied to assignments in the hybrid duplex formed by d(T-C-A-C-A-T) with r(A-U-G-U-G-A). Comparison of intra-residue with inter-residue NOEs yields structural information which suggests that the conformations of both duplexes are similar. The NOEs are consistent with inter-proton distances measured from models of B-form DNA. Circular dichroic results confirm these deductions.