Triazole-modified DNA is a template for in vitro transcription
Oct 3rd 2011, 17:16
In a paper published online in ChemComm, Afaf El-Sagheer and Tom Brown show that a DNA strand containing a triazole phosphodiester mimic is an efficient template for in vitro transcription. This is the first demonstration of transcription through a heavily modified DNA backbone linkage and it suggests that click-ligated DNA could be useful for the direct synthesis of biologically active RNA and proteins.